Product Name :
2′,7′-Dichlorodihydrofluorescein Diacetate (CAS 4091-99-0)
Synonym :
DCFH-DA; DCFH; 2′,7′-Dichlorofluorescin diacetate
Application :
2′,7′-Dichlorodihydrofluorescein Diacetate is a cell-permeable fluorogenic ROS probe
CAS:
4091-99-0
Purity:
Molecular Weight:
487.29
Formula :
C24H16Cl2O7
Physical state:
Solid
solubility :
Soluble in DMSO (25 mg/ml), 100% ethanol (25 mg/ml), DMF (25 mg/ml), and aqueous buffers (sparingly soluble).
Shipping Condition :
Store at -20° C
Melting point:
232° C
SMILES:
CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)O)Cl
References:
:Evidence for free radical formation during the oxidation of 2′-7′-dichlorofluorescin to the fluorescent dye 2′-7′-dichlorofluorescein by horseradish peroxidase: possible implications for oxidative stress measurements. | Rota, C., et al. 1999. Free Radic Biol Med. 27: 873-81. PMID: 10515592Activity of artichoke leaf extract on reactive oxygen species in human leukocytes. | Pérez-García, F., et al. 2000. Free Radic Res. 33: 661-5. PMID: 11200096Is the neutrophil reactive oxygen species production measured by luminol and lucigenin chemiluminescence intra or extracellular? Comparison with DCFH-DA flow cytometry and cytochrome c reduction. | Caldefie-Chézet, F., et al. 2002. Clin Chim Acta. 319: 9-17. PMID: 11922918Flow cytometric measurement of reactive oxygen species production by normal and thalassaemic red blood cells.Val-Cit-PAB-MMAE custom synthesis | Amer, J.1193104-53-8 Order , et al. 2003. Eur J Haematol. 70: 84-90. PMID: 12581189Evaluation of the probes 2′,7′-dichlorofluorescin diacetate, luminol, and lucigenin as indicators of reactive species formation. | Myhre, O., et al. 2003. Biochem Pharmacol. 65: 1575-82. PMID: 12754093Curcumin inhibits UV irradiation-induced oxidative stress and apoptotic biochemical changes in human epidermoid carcinoma A431 cells. | Chan, WH., et al. 2003. J Cell Biochem. 90: 327-38. PMID: 14505349Active oxygen chemistry within the liposomal bilayer. Part IV: Locating 2′,7′-dichlorofluorescein (DCF), 2′,7′-dichlorodihydrofluorescein (DCFH) and 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) in the lipid bilayer. | Afri, M., et al. 2004. Chem Phys Lipids. 131: 123-33. PMID: 15210370Nox4 is critical for hypoxia-inducible factor 2-alpha transcriptional activity in von Hippel-Lindau-deficient renal cell carcinoma. | Maranchie, JK. and Zhan, Y. 2005. Cancer Res. 65: 9190-3. PMID: 16230378The oxidation of 2′,7′-dichlorofluorescin to reactive oxygen species: a self-fulfilling prophesy? | Bonini, MG., et al. 2006. Free Radic Biol Med. 40: 968-75. PMID: 165403922,3,7,8-tetrachlorodibenzo-p-dioxin increases reactive oxygen species production in human endothelial cells via induction of cytochrome P4501A1. | Kopf, PG. and Walker, MK. 2010. Toxicol Appl Pharmacol.PMID:33721054 245: 91-9. PMID: 20171976Effects of H(2)O(2) exposure on human sperm motility parameters, reactive oxygen species levels and nitric oxide levels. | du Plessis, SS., et al. 2010. Andrologia. 42: 206-10. PMID: 20500750A Caco-2 cell-based quantitative antioxidant activity assay for antioxidants. | Wan, H., et al. 2015. Food Chem. 175: 601-8. PMID: 25577125Real time monitoring and quantification of reactive oxygen species in breast cancer cell line MCF-7 by 2′,7′-dichlorofluorescin diacetate (DCFDA) assay. | Figueroa, D., et al. 2018. J Pharmacol Toxicol Methods. 94: 26-33. PMID: 29630935Syzygium cumini extract induced reactive oxygen species-mediated apoptosis in human oral squamous carcinoma cells. | Ezhilarasan, D., et al. 2019. J Oral Pathol Med. 48: 115-121. PMID: 30451321Determination of nitric oxide generation in mammalian neurons using dichlorofluorescin diacetate and flow cytometry. | Gabriel, C., et al. 1997. J Pharmacol Toxicol Methods. 38: 93-8. PMID: 9403780