(10 mL62), high imidazole buffer (ten mL64). The fractions with A280/A325,5.0 were
Chemexpress (10 mL62), high imidazole buffer (ten mL64). The fractions with A280/A325,5.0 have been pooled, dialysed with 100 mM Tris, one hundred mM KCl, pH 7.four and the concentrated PdX protein…