Ture medium (Figure 1).Hu et al. Journal of Translational Medicine 2014, 12:47 http://translational-medicine/content/12/1/Page 4 ofFigure 1 Impact of FTZ on glucose content material in HepG2 cells. HepG2 cells (two ?105 cells/well) have been incubated for 36 h in serum-free DMEM containing 10-6mol/l insulin in the absence or presence of FTZ or RGS. The content of glucose was quantified applying a GOD-POD kit. P0.05 compared to the control cells; *P 0.05 compared to the IR cells.Impact of FTZ on PI-3K p85 mRNA expression in insulinresistant HepG2 cellsTo evaluate the effect of FTZ on PI-3K p85 mRNA expression, total RNA was extracted from HepG2 cells, and real-time PCR was performed. As shown in Figure two, PI3K p85 mRNA expression in HepG2 cells with IR was decreased in comparison with handle cells (P0.(S)-H8-BINAP structure 05 or P0.01). Soon after remedy with FTZ, PI-3K p85 mRNA expression considerably elevated compared to IR cells (P0.05). These results suggest that FTZ induces an insulin sensitizing impact on IR cells by means of the up-regulation of PI-3K p85 mRNA expression in HepG2 cells (Figure 2).Impact of FTZ on IRS1 protein expression in HepG2 cells with insulin resistanceFigure 3 Impact of FTZ on IRS1 protein expression. The protein expression of IRS1 was detected by means of western blotting as described in the text. The figure represents one of 3 experiments with similar benefits. Lane1, control; Lane2, IR (FTZ 0 g/ml); Lane3, RGS 10 mol/l; Lane4, FTZ 100 g/ml; Lane5, FTZ 25 g/ml; Lane 6, FTZ 1 g/ml. P0.05 when compared with the handle cells; *P 0.05 when compared with the IR cells.cells. As shown in Figure three, IRS1 protein expression was drastically lowered in comparison with handle cells (P0.05). Soon after treatment with FTZ, IRS1 protein expression was drastically elevated in comparison with IR cells (P0.05) (Figure 3).Impact of FTZ on body weight of MS ratsAfter the rats had been fed a high-fat diet program for 12 continuous weeks, our outcomes indicated that the physique weight ofTo elucidate the effects of FTZ, western blot analysis was utilized to measure IRS1 protein expression in HepGFigure 2 Effect of FTZ on PI-3K p85 mRNA expression. The expression of PI-3K p85 mRNA was detected via quantitative real-time PCR as described inside the text.Sodium methanesulfinate Purity P 0.PMID:33444667 05 when compared with the control cells; *P 0.05, **P 0.01 when compared with the IR cells.Figure 4 Effect of FTZ on physique weight of MS rats (g). The physique weight was recorded at 0, 4, 8, and 12 weeks. Aspirin (ten ml/kg/day), utilized as a optimistic handle drug, was able to lower body weight of MS rats. Information are imply ?SE (n = 10 for every single group); *P0.05, **P0.01 in comparison to the manage rats; P0.05 in comparison with the MS rats.Hu et al. Journal of Translational Medicine 2014, 12:47 http://translational-medicine/content/12/1/Page five ofFigure 5 (See legend on subsequent page.)Hu et al. Journal of Translational Medicine 2014, 12:47 http://translational-medicine/content/12/1/Page 6 of(See figure on preceding web page.) Figure five Blood biochemical indexes (cholesterol, triglyceride and HDL-C) of MS rats. Blood samples have been collected in the orbital sinus, and cholesterol (a), triglyceride (b), HDL-C (c) levels had been measured following administration of FTZ at six g/kg/day, three g/kg/day or 1.5 g/kg/day, aspirin (AS) at 10 ml/kg/day or saline (manage rats) for eight consecutive weeks in high-fat diet-treated rats. *P0.05, **P0.01 when compared with the manage rats; P0.05, P0.01 in comparison to the MS rats.MS rats was drastically greater than that of control rats (P 0.05, P 0.01). After high, medium or low dosage FTZ treatment for four consecut.