Product Name :
TNF alpha Recombinant Rabbit Monoclonal Antibody [PS01-07]
Predicted band size :
26 kDa
Observed band size :
26/50/17 kDa
Synonyms:
APC1 antibody APC1 protein antibody Cachectin antibody DIF antibody Differentiation inducing factor antibody Macrophage cytotoxic factor antibody Tnf antibody TNF superfamily member 2 antibody TNF superfamily, member 2 antibody TNF, macrophage derived antibody 展开
Function :
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key ‘Ser-418’ residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake.Price of 1196145-01-3 Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance.2072801-99-9 Data Sheet Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.PMID:33744197
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Recombinant protein within full length protein.
Species reactivity:
Human, Mouse
Verify application:
WB
Coupling :
unconjugated
Clone number:
PS01-07
RRID:
AB_3096519
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer solution:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 26 kDa
Positive control :
THP-1 treated with 500ng/mL LPS for 24 hours then add 300ng/mL BFA for 20 hours cell lysate, RAW264.7 treated with 100ng/mL LPS for 7 hours add 300ng/mL BFA for 3 hours cell lysate.