Product Name :
Phospho-IKB alpha (S32) Recombinant Rabbit Monoclonal Antibody [ST53-05]
Predicted band size :
36 kDa
Observed band size :
36 kDa
Synonyms:
I kappa B alpha antibody I-kappa-B-alpha antibody IkappaBalpha antibody IkB-alpha antibody IKBA antibody IKBA_HUMAN antibody IKBalpha antibody MAD 3 antibody MAD3 antibody Major histocompatibility complex enhancer-binding protein MAD3 antibody 展开
Function :
On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IκB family is represented by a protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IκB family member, IκB-ε, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser32 of human IKB alpha.6-Amino-3-bromopicolinonitrile Formula
Species reactivity:
Human, Mouse
Verify application:
WB, IF-Cell, IP
Coupling :
unconjugated
Clone number:
ST53-05
RRID:
AB_3069887
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.1018446-95-1 Chemscene Aliquot store at -20℃ or -80℃.PMID:33749528 Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 36 kDa
Positive control :
HeLa treated with 20ng/mL TNF-α for 5 minutes cell lysate, NIH/3T3 cells were starved for 18 hours then treated with 20 ng/ml TNF alpha for 5 minutes, HeLa.